Proton NMR studies of apo‐neocarzinostatin from Streptomyces carzinostaticus
- 1 June 1990
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 190 (2) , 263-271
- https://doi.org/10.1111/j.1432-1033.1990.tb15571.x
Abstract
The sequence‐specific resonance assignment of apo‐neocarzinostatin from Streptomyces carzinostaticus was carried out from two‐dimensional proton‐NMR spectra. The assignments were obtained for the backbone protons of 111 of the 113 residues of the protein, missing the two CαH of one glycine but including 3 of the 4 prolines. The majority of side chain protons were also assigned. The secondary structure derived from the analysis of sequential connections corresponds to ten β‐strands separated by clearly identified loops and turns. Inter‐strand connectivities and slowly exchanging amide protons confirm the presence of the two disulfide bridges from Cys37 to Cys47 and from Cys88 to Cys93 and indicate a global folding similar to that of the similar proteins, actinoxanthin and macromomycin, for which crystallographic data are available.This publication has 36 references indexed in Scilit:
- Origin of τ2 and τ2 ridges in 2D NMR spectra and procedures for suppressionJournal of Magnetic Resonance (1969), 1986
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Correlation of proton chemical shifts in proteins using two-dimensional double-quantum spectroscopyJournal of Magnetic Resonance (1969), 1983
- Two-dimensional relayed coherence transfer spectroscopy of a proteinJournal of Magnetic Resonance (1969), 1983
- Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteinsBiochemical and Biophysical Research Communications, 1983
- Two-dimensional double quantum 1H NMR spectroscopy of proteinsBiochemical and Biophysical Research Communications, 1983
- Neocarzinostatin chromophore: Presence of a highly strained ether ring and its reaction with mercaptan and sodium borohydrideBiochemical and Biophysical Research Communications, 1983
- Cytotoxic and mutagenic effects of neocarzinostatin in wild-type and repair-deficient yeastsMutation Research Letters, 1982
- Neocarzinostatin chromophore: Presence of a cyclic carbonate subunit and its modification in the structure of other biologically active formsBiochemical and Biophysical Research Communications, 1981
- A two-dimensional nuclear Overhauser enhancement (2D NOE) experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromoleculesBiochemical and Biophysical Research Communications, 1980