Early Activation of Heat Shock Genes in H2O2-Treated Drosophila Cells
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 9 (2) , 147-155
- https://doi.org/10.3109/10715769009148582
Abstract
Drosophila cells of a diploid clone derived from line Kc were treated with 1 mM H2O2, for 1 to 20 minutes. Dot blot and Northern blot analysis of RNAs extracted from control and treated cells showed that the transcriptional activation of the 6 heat-shock genes tested was early, and maximal within 5 minutes of H2O2 treatment. Analysis of the kinetics of induction of the heat-shock proteins (hsps) after an exposure to H2O2 of 2 or 5 minutes, followed by removal, suggests that this brief treatment was sufficient to trigger the synthesis of all the hsps, which was maximal 1.5 to 3h after this short H2O2 treatment.Keywords
This publication has 36 references indexed in Scilit:
- BIOCHEMISTRY OF OXYGEN TOXICITYAnnual Review of Biochemistry, 1989
- Synthesis of stress proteins in rat cardiac myocytes 2-4 days after imposition of hemodynamic overload.Journal of Clinical Investigation, 1988
- The role of oxidative stress in the induction of Drosophila heat-shock proteinsExperimental Cell Research, 1987
- HSP 26 and 27 are phosphorylated in response to heat shock and ecdysterone in Drosophila melanogaster cellsBiochemical and Biophysical Research Communications, 1986
- The Heat Shock ResponsCritical Reviews in Biochemistry, 1985
- Heat shock proteins are induced by cadmium in Drosophila cellsExperimental Cell Research, 1984
- The possible role of the superoxide ion in the induction of heat-shock and specific proteins in aerobic Drosophila cells during return to normoxia after a period of anaerobiosisCanadian Journal of Biochemistry and Cell Biology, 1983
- Genetic regulation during heat shock and function of heat-shock proteins: a reviewCanadian Journal of Biochemistry and Cell Biology, 1983
- Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67BJournal of Molecular Biology, 1983
- Translational efficiency of heat-induced messages in Drosophila melanogaster cellsJournal of Molecular Biology, 1980