Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver
- 1 June 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 171 (3) , 821-824
- https://doi.org/10.1042/bj1710821
Abstract
Rat liver phospholipids were radioactively labeled in vivo before purification of UDP-glucuronyltransferase to homogeneity. The pure enzyme contained very little phospholipid (approx. 0.7 mol of phospholipid/mol of protein). The solubilization detergent Lubrol 12A9 appeared to act as a phospholipid substitute, capable of supporting UDP-glucuronyltransferase activity. Phospholipase C did not inhibit the pure enzyme activity and pure UDP-glucuronyltransferase was stimulated by 40–100% by the addition of phospholipid dispersions.This publication has 21 references indexed in Scilit:
- Phospholipid dependence of UDP-glucuronyltransferaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Purification of UDP‐glucuronyltransferase from untreated rat liverFEBS Letters, 1977
- Separation and partial purification of two differentially inducible UDP-glucuronyltransferases from rat liverBiochemical Pharmacology, 1977
- Latency of epoxide hydratase and its relationship to that of udpglucuronyltransferaseBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Reconstituted liver microsomal enzyme system that hydroxylates drugs, other foreign compounds and endogenous substratesBiochemical and Biophysical Research Communications, 1974
- Pathways of phosphatidylcholine biosynthesis in rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- The kinetics of 32P incorporation into the phospholipids of hepatic rough and smooth microsomal membranes of male and female ratsArchives of Biochemistry and Biophysics, 1970
- Studies on the activation in vitro of glucuronyltranferaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1969
- The use of radioactive choline as a label for microsomal membranes. I. Selectivity of label for endoplasmic reticulum and specificity for lecithinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1968