13C and 2H n.m.r. studies on the biosynthesis of O-methylasparvenone, a hexaketide metabolite of Aspergillus parvulus

Abstract
Incorporations of singly and doubly labelled 13C acetates and [2H3] acetate into O-methylasparvenone, a dihydronaphthalene metabolite of Aspergillus parvulus, and analysis of the resultant enriched samples by high-field 13C and 2H n.m.r. spectroscopy indicate a hexaketide biosynthesis and a novel acetate assembly pattern; the 2H-labelling pattern and levels of enrichment provide information on the sequence and mechanisms of the reduction, oxidation, and deoxygenation steps on the biosynthetic pathway.

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