A System for Pulsed Laser Desorption of Small Biomolecules With Supersonic Jet Injection Using Resonant Two-Photon Ionization (R2Pi) Detection

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Abstract
A system is described that uses laser desorption as a means of volatilizing small, thermally-labile biomolecules into the gas phase as neutral species. The desorbed molecules are then entrapped in a pulsed supersonic jet expansion of CO2 and swept into a differentially pumped time-of-flight mass spectrometer. Laser induced R2PI at 280 nm or 266 nm through the π-π* transition is used to ionize these species softly at modest input energy.