Characterisation of a human bilirubin UDP‐glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures
- 17 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 308 (2) , 161-164
- https://doi.org/10.1016/0014-5793(92)81266-o
Abstract
A cDNA encoding a human bilirubin UDP-glucuronosyltransferase has been isolated and stably expressed in Chinese hamster V79 lung fibroblast cell line. Western blotting of cell homogenates with anti-UGT antibody revealed a highly expressed protein of approx. 55.5 kDa in size. The expressed enzyme specifically catalysed the formation of bilirubin mono- and diglucuronides, and also catalysed the glucuronidation of two phenolic compounds, which are good substrates for other human UGT isoenzymes, at low rates.Keywords
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