The Effect of Glucocorticoid Antagonizing Factor on Hepatoma Cells

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Abstract
Glucocorticoid antagonizing factor (GAF) from cultured macrophages and in serum of endotoxemic mice blocks cortisol induction of phosphoenolpyruvate carboxy-kinase in Reuber H35 rat hepatoma cells. Direct endotoxin treatment of hepatoma cells was not inhibitory. Dibutyryl cyclic AMP induced enzyme synthesis and cortisol induced synthesis of tyrosine aminotransferase were not affected by GAF. Phosphoenolpyruvate carboxykinase induction by cortisol in hepatoma cells could be used to quantitate levels of GAF in serum. This assay system is 10 times more sensitive than in vivo assays for GAF and it can also be used to titrate samples for comparing GAF responses.