The Effect of Oxygen Adaptation on Oxyradical Injury to Pulmonary Endothelium1–3
- 1 July 1983
- journal article
- research article
- Published by American Thoracic Society in American Review of Respiratory Disease
- Vol. 128 (1) , 94-97
- https://doi.org/10.1164/arrd.1983.128.1.94
Abstract
Rats exposed to 85% O2 for 7 days develop an increase in pulmonary tissue levels of antioxidant enzymes and are able to survive subsequent exposure to 100% O2. In order to examine endothelial cell function in O2-adapted rats, we chemically generated oxygen radicals within the pulmonary circulation of lungs isolated from 85% O2-exposed rats and used [14C]5-hydroxytryptamine (5-HT) uptake as an indicator of endothelial cell function. The [14C]5-HT uptake in lungs of O2-exposed rats was similar to that in air-exposed rats and was reduced by cyanide, anoxia, and by imipramine. After generation of O2-derived free radicals within the pulmonary circulation, there was a progressive decline in [14C]5-HT uptake in both groups, with a more rapid decline seen in the lungs of air-exposed rats. These data suggest that pulmonary endothelial cells that survive 85% O2 exposure for 7 days are capable of normal function and are more resistant to further oxyradical injury.This publication has 10 references indexed in Scilit:
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