DNA flow cytometry on human epidermis. The effect of serial biopsy sampling at various times

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Abstract
The effect of serial biopsy sampling on S- and G2 + M-fractions in lower abdominal skin from leg ulcer patients was studied by single-nuclei DNA flow cytometry. Significant differences in both measurements were found in 4 biopsies taken at equal intervals within 24 h, but not in 4 biopsies taken at noon on 4 consecutive days. The variation in S-fractions was closely related to the injury from previous biopsy sampling(s) while the G2 + M-fraction variation tended to follow the time of the day. The study indicated local as well as systemic effects from previous biopsy sampling.