The crystal structure of the three-iron ferredoxin II from Desulfovibrio gigas
- 27 February 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 244 (2) , 447-450
- https://doi.org/10.1016/0014-5793(89)80580-0
Abstract
The crystal structure of oxidized ferredoxin II from the sulfate-reducing bacterium Desulfovibrio gigas has been determined and refined at 1.7 Å resolution. The folding of the polypeptide chain is similar to that of the 2[4Fe-4S] ferredoxin in Peptococcus aerogenes, except for an extended helical segment near the C-terminus. The single [3Fe-4S] cluster in D. gigas is similar to a [4Fe-4S] cluster, but lacks one Fe atom and is coordinated to Cys-8, -14 and -50. The side chain of Cys-11 is not bound to the cluster, but is rotated toward the solvent and modified by some, as yet undetermined, chemical group. Cys-18 and Cys-42 form a disulfide bridge. A previously undetected extra amino acid is found after residue 55.Keywords
This publication has 17 references indexed in Scilit:
- Structure, function and evolution of bacterial ferredoxinsFEMS Microbiology Letters, 1988
- Structure of ferredoxin I from Azotobacter vinelandii.Proceedings of the National Academy of Sciences, 1988
- Evidence for the formation of a ZnFe3S4 cluster in Desulfovibrio gigas ferredoxin IIJournal of the American Chemical Society, 1987
- Spectroscopic studies of the seven-iron-containing ferredoxins from Azotobacter vinelandii and Thermus thermophilusBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1987
- Evidence for the formation of a cobalt-iron-sulfur (CoFe3S4) cluster in Desulfovibrio gigas ferredoxin IIJournal of the American Chemical Society, 1986
- [4Fe-4S]-cluster-depleted Azotobacter vinelandii ferredoxin I: a new 3Fe iron-sulfur protein.Proceedings of the National Academy of Sciences, 1985
- Iron-sulfur stoichiometry and structure of iron-sulfur clusters in three-iron proteins: Evidence for [3Fe-4S] clustersProceedings of the National Academy of Sciences, 1983
- Conversion of [3 Fe–3 S] into [4 Fe–4 S] clusters in a Desulfovibrio gigas ferredoxin and isotopic labeling of iron—sulfur cluster subsitesFEBS Letters, 1982
- Structural control of the redox potentials and of the physiological activity by oligomerization of ferredoxinFEBS Letters, 1978
- Purification, characterization and biological activity of three forms of ferredoxin from the sulfate-reducing bacterium Desulfovibrio gigasBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976