Characterization of HIV-1 vectors with gammaretrovirus envelope glycoproteins produced from stable packaging cells
- 15 January 2004
- journal article
- Published by Springer Nature in Gene Therapy
- Vol. 11 (7) , 591-598
- https://doi.org/10.1038/sj.gt.3302189
Abstract
We have recently described a novel, stable human immunodeficiency virus type 1 (HIV-1) vector packaging system, STAR. High-titre HIV-1 vectors bearing gammaretrovirus envelopes (Env) are continuously produced from STAR cells. Here we compare the properties of such vectors, with the amphotropic murine leukaemia virus (MLV-A) Env, a modified gibbon ape leukaemia virus (GALV) Env and two modified versions of the cat endogenous retrovirus RD114 Env, produced from STAR cells, to transiently produced HIV-1 vectors with vesicular stomatitis virus G protein (VSV-G). Our results indicate that gammaretrovirus pseudotypes from STAR cells are relatively stable at 37°C and are resistant to inactivation by freeze/thaw cycling or incubation with human sera. HIV-1(VSV-G) was, however, sensitive to freeze/thaw when harvested in serum-free media and was readily inactivated in human sera. Furthermore, the titre of 'gamma-retrovirus' pseudotypes, but not HIV-1(VSV-G), could be increased by the use of a combination of polybrene and spinoculation. All pseudotypes could be efficiently concentrated, but soluble gammaretrovirus Env could act as an inhibitor of infection.Keywords
This publication has 46 references indexed in Scilit:
- Continuous high-titer HIV-1 vector productionNature Biotechnology, 2003
- Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primatesBlood, 2002
- Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus VectorsJournal of Virology, 2001
- Generation of a Flexible Cell Line with Regulatable, High-Level Expression of HIV Gag/Pol Particles Capable of Packaging HIV-Derived VectorsMolecular Therapy, 2001
- A Stable System for the High-Titer Production of Multiply Attenuated Lentiviral VectorsMolecular Therapy, 2000
- Brief Report: Analysis of 4070A Envelope Levels in Retroviral Preparations and Effect on Target Cell Transduction EfficiencyHuman Gene Therapy, 2000
- Efficient Gene Transfer into Primary Human CD8+T Lymphocytes by MuLV-10A1 Retrovirus PseudotypeHuman Gene Therapy, 2000
- Gene Therapy of Human Severe Combined Immunodeficiency (SCID)-X1 DiseaseScience, 2000
- Retrovirus-Mediated Gene Transfer into Human CD34+38lowPrimitive Cells Capable of Reconstituting Long-Term CulturesIn Vitroand Nonobese Diabetic–Severe Combined Immunodeficiency MiceIn VivoHuman Gene Therapy, 1998
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996