THE OXIDATION OF TRYPTAMINE BY RAT LIVER AND OTHER TISSUE SUSPENSIONS

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Abstract
Tryptamine solutions were incubated with crude suspensions of rat and guinea pig tissues in the Bareroft-Warburg apparatus. O2 consumption was measured. Solutions incubated for various periods of time were deprotein-ized with perchloric acid, and the supernatant fraction was examined in the Beckman DU spectrophotometer. Consistent and marked changes were found in the UV absorption spectrum of the tryptamine solutions. The quantitative changes were utilized for the measurement of monoamine oxidase activity by the "substrate disappearance" method. The significance of the qualitative changes in absorption spectrum is discussed. Similar experiments with 5-hydroxytryptamine are presented. This substrate is oxidized by rat liver at about the same rate as tryptamine.

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