USE OF EXPRESSED SAP IN PHYSIOLOGIC STUDIES OF CORN

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Abstract
The amount of sap expressed from corn tissue under a uniform pressure and with a uniform time for draining the cage was influenced by the preliminary treatment of the tissue, by the size of sample, and by the kind of tissue and its moisture content as affected by its stage of development. The total solids content differed in successive portions of sap expressed from corn tissue, the size of the differences varying with the treatment preliminary to expression, the kind of tissue and its moisture content. The sucrose and free reducing sugars, reported together here as total sugars, were constant for successive portions of sap expressed after grinding the tissue, but decreased in successive portions from minced tissue. The percentages of total sugars in three kinds of fresh corn tissue from two varieties, at different stages of growth. as determined by the standard method and from the sap expressed after grinding, were in excellent agreement, the coefficient of correlation being 0.9913. The values by the two methods were in as good agreement as those for 12 pairs of samples for which sugars were determined by the standard method. Expressing the sap from a 100-gram sample of ground fresh corn tissue under a pressure of 5,000 pounds per square inch, at the same time forcing the sap through a filter, and allowing a uniform time (5 minutes) for draining, provided samples of sap that were satisfactory for certain physical and chemical examinations.

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