CBP/p300 and muscle differentiation: no HAT, no muscle
Open Access
- 3 December 2001
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 20 (23) , 6816-6825
- https://doi.org/10.1093/emboj/20.23.6816
Abstract
Terminal differentiation of muscle cells follows a precisely orchestrated program of transcriptional regulatory events at the promoters of both muscle‐specific and ubiquitous genes. Two distinct families of transcriptional co‐activators, GCN5/PCAF and CREB‐binding protein (CBP)/p300, are crucial to this process. While both possess histone acetyl‐transferase (HAT) activity, previous studies have failed to identify a requirement for CBP/p300 HAT function in myogenic differentiation. We have addressed this issue directly using a chemical inhibitor of CBP/p300 in addition to a negative transdominant mutant. Our results clearly demonstrate that CBP/p300 HAT activity is critical for myogenic terminal differentiation. Furthermore, this requirement is restricted to a subset of events in the differentiation program: cell fusion and specific gene expression. These data help to define the requirements for enzymatic function of distinct coactivators at different stages of the muscle cell differentiation program.Keywords
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