A Transport Form of Collagen from Embryonic Tendon: Electron Microscopic Demonstration of an NH 2 -Terminal Extension and Evidence Suggesting the Presence of Cystine in the Molecule
- 1 January 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (1) , 60-64
- https://doi.org/10.1073/pnas.69.1.60
Abstract
When cells were isolated from chickembryo tendons and incubated in vitro for 2-6 hr, essentially all the newly-synthesized collagen was recovered from the incubation medium as a transport form larger than tropocollagen. Experiments in which cells were incubated with [(14)C]cystine suggested that the transport form contained cystine and that it was, in part, stabilized by disulfide bonds. Electron microscopy of segment-long-spacing aggregates prepared from the transport form of collagen showed that the native molecule differed from tropocollagen in that it had an extension of about 13 nm (130 A) at the NH(2)-terminal end.Keywords
This publication has 10 references indexed in Scilit:
- Further evidence for a transport form of collagen. Its extrusion and extracellular conversion to tropocollagen in embryonic tendonFEBS Letters, 1971
- Studies on protocollagen: Identification of a precursor of proto α1Biochemical and Biophysical Research Communications, 1971
- The binding of detergents to proteins. I. The maximum amount of dodecyl sulfate bound to proteins and the resistance to binding of several proteins.1971
- Inhibition of proline and lysine hydroxylation prevents normal extrusion of collagen by 3T6 fibroblasts in cultureFEBS Letters, 1971
- Evidence for Procollagen, a Biosynthetic Precursor of CollagenProceedings of the National Academy of Sciences, 1971
- The Nature of the Collagen Synthesized by Cultured Human FibroblastsProceedings of the National Academy of Sciences, 1971
- The Gross Conformation of Protein-Sodium Dodecyl Sulfate ComplexesJournal of Biological Chemistry, 1970
- Studies on the specificity of bacterial collagenaseBiochemical and Biophysical Research Communications, 1970
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- Specific degradation of the collagen molecule by tadpole collagenolytic enzyme.Proceedings of the National Academy of Sciences, 1965