Molecular cloning and functional expression of the second mouse nm23/NDP kinase gene, nm23‐M2
Open Access
- 14 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 309 (3) , 358-362
- https://doi.org/10.1016/0014-5793(92)80807-s
Abstract
A new murine cDNA of nm23/NDP kinase was isolated. A RT‐PCR product was obtained from the normal mouse liver mRNA with primers designed for the human nm23‐H2 gene. The product was used as a probe to screen a cDNA library from the murine melanoma cell line. B16, and two clones containing the entire open reading frame were obtained. It was predicted that the DNA sequence encoded 152 amino acids which was 98% identical to the nm23‐H2 protein. The entire nm23‐M1 and ‐M2 gene‐coding regions were translated as fusion proteins with a glutathione S‐transferase. These fusion proteins displayed NDP kinase activities.Keywords
This publication has 10 references indexed in Scilit:
- Integrins: Versatility, modulation, and signaling in cell adhesionCell, 1992
- Identity of a differentiation inhibiting factor for mouse myeloid leukemia cells with NM23/nucleoside diphosphate kinaseBiochemical and Biophysical Research Communications, 1992
- Nucleoside diphosphate kinase from Escherichia coli; its overproduction and sequence comparison with eukaryotic enzymesGene, 1991
- Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzymeJournal of Biological Chemistry, 1991
- Isolation and characterization of a cDNA clone encoding rat nucleoside diphosphate kinase.Journal of Biological Chemistry, 1990
- Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum.Journal of Biological Chemistry, 1990
- Nucleoside diphosphate kinase from Myxococcus xanthus. I. Cloning and sequencing of the gene.Journal of Biological Chemistry, 1990
- Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila.Genes & Development, 1988
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- Evidence for a Novel Gene Associated With Low Tumor Metastatic PotentialJNCI Journal of the National Cancer Institute, 1988