Biosynthesis of vitamin B12: structural studies on precorrin-8x, an octamethylated intermediate and the structure of its stable tautomer

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Abstract
The structure of precorrin-8x, the octamethylated B12-intermediate which follows precorrin-6y in Pseudomonas denitrificans, is largely determined by 13C-labelling and NMR spectroscopy and the structure of its most stable tautomer is established.