Über die Spaltung von Lichenin und Cellulose durch die Fermente des Gerstenmalzes.

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Abstract
Lichenin and cellulose are hydrolyzed to glucose by the same enzymes, the reaction involving 3 successive steps. Active lichenase was prepared from green malt by fermenting the malt extract with yeast to remove reducing substances. The enzyme was then adsorbed on Al (OH)3 in acid solution, followed by elu-tion in alkaline solution. This preparation was 2.3 times as active as the original malt extract. Hydrolysis at first obeys the law of monomolecular reaction, and later follows the Shutz rule. Proportionality of enzyme concentration to rate and degree of hydrolysis varied with age and purity of enzyme preparation. Old pre-parations could be partially revived by warming. Hy-drolysis proceeded at a greater rate, and to a greater degree, with the more highly purified white preparations than with the less pure gray ones. Portions of cellulose were dispersed by Guignet''s H2SO4, Von Weimarn''s LiCl, and Ca (SCN)2 methods. After complete removal of the dispersing agents, the cellulose which had been dispersed by Ca (SCN)2 was least resistant to hydrolysis by the barley malt enzymes. The Guignet cellulose was the most resistant of the 3.

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