Amino acid oxidation and alanine production in rat hemidiaphragm in vitro. Effects of dichloroacetate
- 1 October 1984
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 223 (1) , 113-117
- https://doi.org/10.1042/bj2230113
Abstract
Dichloroacetate (an activator of pyruvate dehydrogenase) stimulates 14CO2 production from [U-14C]glucose, but not from [U-14C]glutamate, [U-14C]aspartate, [U-14C]-and [1-14C]-valine and [U-14C]- and [1-14C]-leucine. Pyruvate dehydrogenase is not rate-limiting in the oxidation to CO2 of amino acids that are metabolized to tricarboxylic acid-cycle intermediates, and carbohydrate (and not amino acids) is the main carbon precursor in alanine formation in muscle.This publication has 16 references indexed in Scilit:
- Muscle alanine synthesis and hepatic gluconeogenesisBiochemical Society Transactions, 1980
- Studies on the regulation of leucine catabolismArchives of Biochemistry and Biophysics, 1980
- Carboxylation and decarboxylation reactions. Anaplerotic flux and removal of citrate cycle intermediates in skeletal muscle.Journal of Biological Chemistry, 1979
- Glucose and Amino Acid Metabolism in Perfused Skeletal Muscle: Effect of DichloroacetateDiabetes, 1978
- The origin of alanine produced in skeletal muscle.Journal of Biological Chemistry, 1978
- The release of alanine by rat diaphragm muscle in vitroBiochemical Journal, 1977
- The formation of alanine from amino acids in diaphragm muscle of the ratBiochemical Journal, 1976
- Alanine and glutamine synthesis and release from skeletal muscle. IV. beta-Adrenergic inhibition of amino acid release.Journal of Biological Chemistry, 1976
- Amino Acid Metabolism in ManAnnual Review of Biochemistry, 1975
- Effects of dichloroacetate on the metabolism of glucose, pyruvate, acetate, 3-hydroxybutyrate and palmitate in rat diaphragm and heart muscle in vitro and on extraction of glucose, lactate, pyruvate and free fatty acids by dog heart in vivoBiochemical Journal, 1973