Making ends meet in genetic analysis using padlock probes
Open Access
- 18 March 2002
- journal article
- review article
- Published by Hindawi Limited in Human Mutation
- Vol. 19 (4) , 410-415
- https://doi.org/10.1002/humu.10073
Abstract
Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single‐nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling‐circle replication, as well as to sort reaction products on tag‐arrays. We argue that the probes have the potential to render high‐throughput genetic analyses precise and affordable. Hum Mutat 19:410–415, 2002.Keywords
Funding Information
- Beijer Foundation
- Medical and Technological Science Councils of Sweden
- Swedish Cancer Fund
- Foundation for Medical Research in Uppsala
- Polysackaridforskning i Uppsala AB
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