DNA Deaminating Ability and Genotoxicity of Nitric Oxide and its Progenitors
- 15 November 1991
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5034) , 1001-1003
- https://doi.org/10.1126/science.1948068
Abstract
Nitric oxide (NO), a multifaceted bioregulatory agent and an environmental pollutant, can also cause genomic alterations. In vitro, NO deaminated deoxynucleosides, deoxynucleotides, and intact DNA at physiological pH. That similar DNA damage can also occur in vivo was tested by treating Salmonella typhimurium strain TA1535 with three NO-releasing compounds, including nitroglycerin. All proved mutagenic. Observed DNA sequence changes were >99% C→T transitions in the his G46 (CCC) target codon, consistent with a cytosine-deamination mechanism. Because exposure to endogenously and exogenously produced NO is extensive, this mechanism may contribute to the incidence of deamination-related genetic disease and cancer.Keywords
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