Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L.

Abstract
Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules [caused by infection with Rhizobium leguminosarum] of the garden pea (P. sativum L., cv. ''Little Marvel''). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on TLC in 3 solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found.