RADIOIMMUNOASSAY FOR SERUM TRANSCOBALAMIN-II

  • 1 January 1977
    • journal article
    • research article
    • Vol. 89  (3) , 645-652
Abstract
A radioimmune assay for transcobalamin II (TC II) was devised from the following: TC II-57Co B12 precipitated from normal serum with (NH4)2SO4 as the labeled ligand; the TC II of whole serum as the standard source of TC II; rabbit anti-pure TC II as the binding agent and separation of the bound and free TC II-B12 by precipitation of the antibody bound with polyethylene glycol. The assay was responsive to either TC II or TC II-B12 and to TC II either pure or in crude preparations. It was not responsive to R-type binders of B12. The median TC II of 10 normal [human] sera was 890 pg/ml and of 10 sera from 10 random hospital patients was 1010 pg/ml. There was no measurable TC II in the serum of a child with congenital absence of TC II and the assay measured levels greater than 5000 pg/ml in abnormalities of TC II metabolism.

This publication has 1 reference indexed in Scilit: