Abstract
Ten male Holstein calves, 16 weeks of age, were slaughtered at time intervals after last feeding which varied from 2 to 12 hours. Concentrations of thiamine, riboflavin and niacin were determined in the contents of the digestive tracts and compared with those in the feed eaten. Increased levels of all three vitamins were found with individual exceptions in the case of certain calves with respect to thiamine and niacin. Certain differences due to the time interval between feeding and slaughter were noted, but, due to the variability between individual animals, little importance could be attached to these differences. Therefore, under the conditions of this experiment it appeared that the time interval between last feeding and slaughter had little effect on the levels of the three vitamins in the rumen contents or in the contents of other parts of the digestive tract.

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