Comparison of Molecular and Conventional Methods for Typing of Enteroviral Isolates
Open Access
- 1 March 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (3) , 1069-1070
- https://doi.org/10.1128/jcm.40.3.1069-1070.2002
Abstract
Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.Keywords
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