Acid secretion in fetal rat stomach in vitro

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Abstract
In vivo fetal rat stomach produces HCl 48 h before birth. This study examines the mechanisms of H+ secretion from days 19-21 before birth. Isolated fetal stomachs were mounted as flat sheets in Ussing chambers for measurement of the transepithelial H+ fluxes (JH+) and short-circuit current (Isc), as indexes of the active ionic fluxes, and for measurement of total ionic conductance (G) and unidirectional mannitol fluxes from serosa to mucosa .**GRAPHIC**. as indexes of passive permeability. JH+ was absent at day 19 but reached 0.75 .+-. 0.1 and 0.75 .+-. 0.09 .mu.eq .cntdot. h-1 .cntdot. cm-2 at days 20 and 21, respectively. Concomitantly, Isc increased significantly (56%) between days 19 and 20 in the direction of anion secretion or cation absorption. Parallel reductions in G (45%) and in .**GRAPHIC**. (66%) were observed between days 19 and 20. The simultaneous appearance of active H+ secretion and decreased passive transepithelial permeability strongly suggests that both processes are involved in the mechanism of acidification of the fetal rat stomach before birth.

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