Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates

1 May 1995

journal article
research article
Published by American Society for Microbiology in Journal of Clinical Microbiology

Vol. 33 (5) , 1383-4
https://doi.org/10.1128/jcm.33.5.1383-1384.1995

Abstract

We describe a rapid method for subtyping Mycobacterium tuberculosis based on PCR amplification of segments located between two distinct DNA repetitive elements. This method, double-repetitive-element PCR, classified 46 clinical isolates as having 25 distinct patterns; the conventional restriction fragment length polymorphism analysis classified the same isolates as having 23 distinct patterns. The double-repetitive-element PCR is a rapid subtyping method that has a discriminating power similar to that of the restriction fragment length polymorphism method.

Keywords

This publication has 1 reference indexed in Scilit:

IS6110: Conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting
Molecular and Cellular Probes, 1991