T CELL-FUNCTION DETECTED IN MURINE BONE-MARROW CELLS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 47  (1) , 15-24
Abstract
Mouse bone marrow cells were fractionated by BSA [bovine serum albumin] discontinuous density gradient centrifugation and a small lymphocyte rich fraction was obtained at the high density. Cells of this fraction responded in vitro to T [thymus-derived] cell mitogens and alloantigens. They were also able to mount a graft vs. host reaction when assessed by spleen weight assay and by the method of inhibiting erythroid cell growth by allogeneic lymphoid cells. These lymphocytes possess T cell functions. They carry only little .theta. antigen, assessed by the cytotoxic test and by the absorption test. The amount of .theta. antigen on a cell might not correlate with T cell functions; these lymphocytes might be mature ones in the course of postthymic maturation. Hemopoietic stem cells were determined by spleen colony formation and the peak of colony-forming efficiency was seen at the low density. Immunocompetent cells causing GVHR can probably be separated from hemopoietic stem cells. This procedure may be applied for prevention and reduction of GVHR in allogeneic bone marrow transplantation in humans.

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