Monoclonal Antibodies to Escherichia coli β-Galactosidase and Their Use for Detection and Purification of Recombinant Expression Products
- 1 June 1992
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 11 (3) , 385-390
- https://doi.org/10.1089/hyb.1992.11.385
Abstract
A panel of seven mouse monoclonal antibodies (BG-01-BG-07) was prepared against β-galactosidase derived from E. coli. The antibodies are β-galactosidase specific, show no cross-reactivity with other E. coli proteins and can be used for identification and characterization of β-galactosidase fusion proteins expressed in lambda expression vectors. One of the antibodies allows a simple, one-step isolation of the fusion proteins directly from the crude bacterial lysates using immunoaffinity chromatography.Keywords
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