A Micro-method for the Enzymatic Determination of Acetoacetate and 3-Hydroxybutyrate in Blood and Urine
- 1 January 1970
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 25 (2) , 171-179
- https://doi.org/10.3109/00365517009049200
Abstract
An enzymatic micro-method for the determination of acetoacetate and 3-hydroxybutyrate in blood and urine is described. 150 μl blood or urine is needed for the analysis of each ketone body. Interference was not found from lactate, malate, pyruvate, or oxaloacetate with either the acetoacetate or 3-hydroxybutyrate determination. The addition of lactate or malate dehydrogenase is therefore unnecessary. The significance of the pH of the reaction mixture is discussed. The analysis is well-suited for routine use.Keywords
This publication has 4 references indexed in Scilit:
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- A fluorimetric procedure for the determination of ketone bodies in very small quantities of bloodClinica Chimica Acta; International Journal of Clinical Chemistry, 1966
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