Evidence for receptor‐linked activation of phospholipase D in rat parotid glands Stimulation by carbamylcholine, PMA and calcium

17 December 1992

journal article
Published by Wiley in FEBS Letters

Vol. 314 (3) , 489-492
https://doi.org/10.1016/0014-5793(92)81533-r

Abstract

In order to test if phospholipase D (PLD) activity exists in the rat parotid gland, we took advantage of the fact that, in the presence of ethanol, PLD generates phosphatidylethanol (PEth) via a transphosphatidylation reaction. Lipid extracts of parotid acini prelabelled with [³H]myristic acid were analyzed by thin layer chromatography to determine [³H]phosphatidylethanol ([³H]PEth) formation. Carbamylcholine (1 mM) stimulated [³H]PEth formation in the presence of 2% ethanol, this effect was completely inhibited by atropine (10 μM), PMA (0.1–1 μM) and ionomycine (10 μM) also caused [³H]PEth generation. We conclude that a phospholipase D activity is present in the rat parotid gland and is regulated by muscarinic cholinergic receptors. Protein kinase C and calcium could also modulate this activity. This report provides the first evidence for the existence and receptor-linked regulation of phospholipase D in an exocrine gland, the rat parotid gland.

Keywords

This publication has 24 references indexed in Scilit:

fMet-Leu-Phe-induced activation of phospholipase D in human neutrophils. Dependence on changes in cytosolic free Ca2+ concentration and relation with respiratory burst activation.
Published by Elsevier ,1991
Production of phosphatidylethanol by phospholipase D phosphatidyl transferase in intact or dispersed pancreatic islets: Evidence for the in situ metabolism of phosphatidylethanol
Archives of Biochemistry and Biophysics, 1990
Phorbol ester treatment of intact rabbit platelets greatly enhances both the basal and guanosine 5′-[γ-thio]triphosphate-stimulated phospholipase D activities of isolated platelet membranes. Physiological activation of phospholipase D may be secondary to activation of phospholipase C
Biochemical Journal, 1990
A Potential Role for Phospholipase-D in the Angiotensin-II-Induced Stimulation of Aldosterone Secretion from Bovine Adrenal Glomerulosa Cells*
Endocrinology, 1990
The regulation and cellular functions of phosphatidylcholine hydrolysis
Biochemical Journal, 1990
Biochemical characterization of phospholipase D activity from human neutrophils
European Journal of Biochemistry, 1989
Phosphatidylcholine Hydrolysis by Phospholipase D Determines Phosphatidate and Diglyceride Levels in Chemotactic Peptide-stimulated Human Neutrophils
Published by Elsevier ,1989
Phospholipase D in homogenates from HL-60 granulocytes: Implications of calcium and G protein control
Biochemical and Biophysical Research Communications, 1989
Gonadotropin-releasing hormone activates phospholipase D in ovarian granulosa cells
Published by Elsevier ,1989
The formation of phosphatidylglycerol and other phospholipids by the transferase activity of phospholipase D
Biochemical Journal, 1967