Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP‐dependent protein kinase
- 15 December 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 209 (2) , 219-222
- https://doi.org/10.1016/0014-5793(86)81114-0
Abstract
We report here the isolation and sequence of a cDNA for the type II regulatory subunit of the cAMP‐dependent protein kinase (cAMP‐PK) from a λgt‐11 cDNA library derived from a porcine epithelial cell line (LLC‐PK1). The cDNA was detected by immunological screening using an affinity purified polyclonal antibody for bovine RII. DNA sequence analysis of the 467 bp EcoRI insert confirmed the identity of the clone, because the deduced amino acid sequence corresponded to the published sequence for the bovine RΠ protein. Northern analysis of total RNA from the LLC‐PK1 cells indicated a single mRNA species of about 6.0 kb, probably derived from a single copy gene.Keywords
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