Atropine-resistant depolarization in the guinea-pig small intestine

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Abstract
Junction potentials were recorded from the circular muscle cells of the guinea pig ileum following transmural stimulation in the presence of atropine at 30.degree. C. Single stimuli produced a transient hyperpolarization, the inhibitory junction potential (IJP). At high stimulus strengths the IJP was followed by a post-stimulus depolarization (PSD). During repetitive stimulation the magnitude of the hyperpolarization decreased; at the end of the stimulus period the PSD was enhanced and often reached threshold for the generation of action potentials. The size of the PSD was not directly related to the degree of the preceding hyperpolarization. Hyperpolarization of the circular muscle cells was produced by the application of anodal current using large external electrodes. Rapid cessation of the applied current produced a transient after-depolarization which was shorter in time course than the PSD following the IJP. If the applied anodal current was reduced slowly (at a rate which mimicked the decrease in the hyperpolarization during repetitive nerve stimulation) no after-depolarization was observed. Conditioning hyperpolarization of the circular muscle cells reduced the amplitude of the IJP. The IJP was reversed at membrane potentials greater than approximately -90 mV. The PSD did not appear to be due to the extracellular accumulation of potassium ions following the IJP since the PSD persisted even when the IJP was reversed. The neurotoxin apamin reversibly abolished the IJP and unmasked a transient excitatory junction potential (EJP) with a variable latency (350-900 ms).