Induction of unscheduled DNA synthesis by chemical mutagens in testicular cells of the mouse in vitro
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Archives of Toxicology
- Vol. 37 (3) , 195-201
- https://doi.org/10.1007/bf00355488
Abstract
Testicular cells of male mice were isolated, and the incorporation of tritium labeled thymidine into the DNA of the cells was estimated after exposure to various chemical mutagens. The normal semiconservative DNA synthesis was suppressed by the addition of hydroxyurea. Thirteen compounds were tested. Unscheduled DNA synthesis (UDS) was stimulated by ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) and triaziquone, and, to a lesser extent, by 4-nitroquinoline 1-oxide (4-NQO), mitomycin C, ICR-191 and nitrogen mustard (HN-2). No thymidine incorporation could be estimated after incubation of the cells with 9 aminoacridine, hydroxyurethane, α-naphthylamine, 2-acetylaminofluorene (AAF) nor with N-hydroxy-2-acetylaminofluorene (N-OH-AAF). Eine Zellsuspension aus dem Hoden der Maus wurde gewonnen und mit verschiedenen chemischen Mutagenen inkubiert. Dabei wurde die semikonservative DNA-Synthese durch Zugabe von Hydroxyharnstoff gehemmt. Die durch die Einwirkung der mutagenen Agentien hervorgerufene DNA-Reparatursynthese wurde durch Messung des Einbaus von Tritium-markiertem Thymidin in die Zellen bestimmt. Dreizehn Substanzen wurden untersucht. Eine deutliche DNA-Reparatursynthese wurde durch Äthylmethansulfonat (EMS), Methylmethansulfonat (MMS), N-Methyl-N′-nitro-N-nitrosoguanidin (MNNG) und Trenimon stimuliert. 4-Nitrochinolin-1-oxid (4-NQO), Mitomycin C, ICR-191 und N-Lost (HN2) zeigten einen schwächeren Effekt. Kein Einbau von markiertem Thymidin konnte nach der Inkubation mit 9-Aminoacridin, Hydroxyurethan, α-Naphthylamin, 2-Acetylaminofluoren (AAF) und N-Hydroxy-2-acetylaminofiuoren (NOH-AAF) festgestellt werden.This publication has 11 references indexed in Scilit:
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