Production of Radioactive Mosquitoes

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Abstract
Second-instar larvae of Aedes aegypti reared in dist. water containing 0.05-10 [mu]c /ml. of Na2HP32O4 were greatly retarded in development, particularly at the higher concns.; in 1[mu]c /ml. concn. adults emerged in 15 days, and showed a marked accumulation of radiophosphorus, activity per adult ranging from 6761 to 10,132 counts/min. Third and early 4th instar larvae placed in a 10 [mu]c /ml. concn. of the radioactive salt developed normally, and emerged from the 6th to 10th day; radioactivity of these adults averaged 29,838 counts/min. One-day-old pupae placed in 10[mu]c /ml. concn. emerged in 24-48 hrs. and averaged only 30 counts/min. activity. Adults emerging from larvae which had been reared in soln. containing 10 [mu]c /ml. for 8 days, rinsed 10 times in dist. water, and kept in dist. water until emerged, showed an avg. activity of 27,778 counts/min., thus indicating that the radioactivity was not due to surface contamination of adults with rearing medium.

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