Erythropoietin Induced Ultrastructural Alterations to J2E Cells and Loss of Proliferative Capacity with Terminal Differentiation
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Growth Factors
- Vol. 9 (4) , 317-328
- https://doi.org/10.3109/08977199308991592
Abstract
Erythropoietin (epo) induced differentiation of the J2E erythroid cell line is characterized by haemoglobin synthesis, together with morphological changes and an immediate increase in proliferation. In this manuscript we have shown that the size of J2E cells decreased during differentiation and the nucleus to cytoplasm ratio was reduced appreciably. Furthermore, major ultrastructural alterations occurred-mitochondria, rough endoplasmic reticulum and Golgi apparatus decreased in size and number with maturation, while nuclei condensed considerably before extrusion. The use of mitotic indices, 3H-thymidine uptakes and flow cytometry confirmed that the immature J2E cells undergo enhanced replication shortly after epo stimulation. In addition, we demonstrated that cell division ceased as the cells entered the final stages of erythroid differentiation. Thus the J2E line provides a useful model, not only for haemoglobin synthesis, but for all aspects of erythroid terminal differentiation.Keywords
This publication has 19 references indexed in Scilit:
- Erythropoietin-induced stimulation of differentiation and proliferation in J2E cells is not mimicked by chemical inductionBlood, 1992
- Chromatin as an essential part of the transcriptional mechanimNature, 1992
- Erythropoietin Retards DNA Breakdown and Prevents Programmed Death in Erythroid Progenitor CellsScience, 1990
- In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin.Proceedings of the National Academy of Sciences, 1988
- Control of globin gene transcription by erythropoietin in erythroblasts from friend virus-infected mice.Molecular and Cellular Biology, 1985
- Rapid hypotonic method for flow cytofluorometry of monolayer cell cultures. Some pitfalls in staining and data analysis.Journal of Histochemistry & Cytochemistry, 1978
- Separation of the erythropoietin-responsive progenitors BFU-E and CFU-E in mouse bone marrow by unit gravity sedimentationBlood, 1976
- Ultrastructure of Human Bone Marrow Cell MaturationPublished by Elsevier ,1976
- The Effect of Erythropoietin on Colonial Growth of Erythroid Precursor Cells In VitroProceedings of the National Academy of Sciences, 1974
- The ultrastructure of erythroblastic islands and reticular cells in mouse bone marrowJournal of Ultrastructure Research, 1967