Expression of Plasmids Coding for Colonization Factor Antigen II (CFA/II) and Enterotoxin Production in Escherichia coli

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Abstract
Two plasmids transferred from enterotoxigenic E. coli of serotype 06. H16 and biotypes A and C coded for mannose-resistant hemagglutination (MRHA) and production of heat-stable and heat-labile enterotoxin. Both plasmids were non-autotransferring, being mobilized most efficiently by the R plasmid R100-1. They were similar in their genetic properties, incompatible with each other and plasmids of the Inc group FI. The wild-type strains produced the colonization factor antigen II which was made up of different coli surface antigens (CS). The biotype A strains produced CS1 and CS3; the biotype C strains produced CS2 and CS3. These 3 antigens caused MRHA. When plasmids coding for MRHA were transferred to K12 strains, the degree of hemagglutination was markedly reduced; only CS3 was produced. When both plasmids were transferred back into biotype A strains, good MRHA was restored and the strains produced CS1 and CS3. In a biotype C strain CS2 and CS3 were formed. The production of the antigens was compared by enzyme-linked immunosorbent assays. The strains were also examined by EM CS1 and CS2 but not CS3, were found to be were fimbrial antigens.

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