Molecular cloning and expression of a bovine endothelial inward rectifier potassium channel1
Open Access
- 9 June 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 409 (2) , 277-282
- https://doi.org/10.1016/s0014-5793(97)00514-0
Abstract
A 5.1 kb cDNA encoding an inward rectifier K+ channel (BIK) was isolated from a bovine aortic endothelial cell library. The cDNA codes for a 427‐amino‐acid protein with two putative transmembrane regions. Sequence analysis reveals that BIK is a member of the Kir2.1 family of inward rectifier K+ channels. Expression in Xenopus oocytes showed that BIK is a K+‐specific strong inward rectifier channel that is sensitive to extracellular Ba2+, Cs+, and a variety of anti‐arrhythmic agents. Northern analysis revealed that endothelial cells express a 5.5 kb BIK mRNA that is sensitive to shear stress.Keywords
This publication has 51 references indexed in Scilit:
- Subunit-specific inhibition of inward-rectifier K+channels by quinidineFEBS Letters, 1995
- Gating of inwardly rectifying K+ channels localized to a single negatively charged residueNature, 1994
- Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brainFEBS Letters, 1994
- Shear-dependence of endothelial functionsCellular and Molecular Life Sciences, 1994
- Pulsatile Velocity Measurements in a Model of the Human Abdominal Aorta Under Simulated Exercise and Postprandial ConditionsJournal of Biomechanical Engineering, 1994
- Intracellular calcium, currents, and stimulus-response coupling in endothelial cells.Hypertension, 1993
- Membrane ion channels of endothelial cellsTrends in Pharmacological Sciences, 1992
- Bradykinin-induced potassium current in cultured bovine aortic endothelial cellsThe Journal of Membrane Biology, 1990
- Voltage-gated and receptor-mediated ionic currents in the membrane of endothelial cellsJournal of Molecular and Cellular Cardiology, 1989
- Potassium current and the effect of cesium on this current during anomalous rectification of the egg cell membrane of a starfish.The Journal of general physiology, 1976