Dynamic behavior of transcription factors on a natural promoter in living cells
Open Access
- 1 December 2002
- journal article
- research article
- Published by Springer Nature in EMBO Reports
- Vol. 3 (12) , 1188-1194
- https://doi.org/10.1093/embo-reports/kvf244
Abstract
Through the use of photobleaching techniques, we examined the dynamic interaction of three members of the transcrsiption apparatus with a target promoter in living cells. The glucocorticoid receptor (GR) interacting protein 1 (GRIP‐1) exhibits a half maximal time for fluorescent recovery (τR) of 5 s, reflecting the same rapid exchange as observed for GR. In contrast, the large subunit (RPB1) of RNA polymerase II (pol II) required 13 min for complete fluorescence recovery, consistent with its function as a processive enzyme. We also observe a complex induction profile for the kinetics of GR‐stimulated transcription. Our results indicate that GR and GRIP‐1 as components of the activating complex are in a dynamic equilibrium with the promoter, and must return to the template many times during the course of transcriptional activation.Keywords
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