Grass Pollen Extracts IV. The Biological Activity of Rye Pollen Extracts and their Fractions

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Abstract
Testing by prick on the skin of sensitive subjects showed (1) that aqueous extraction removes most of the reactive material from the pollen; (2) that little or no active material is lost by dialysis of aqueous extract; and (3) that reactivity was not restricted to any single fraction prepared by Johnson and Thorne. Immune sera were obtained in rabbits against 3 fractions produced by electrophoresis. By precipitation, agglutination with antigen-coated tanned erythrocytes and inhibition tests, it was shown that at least 3 antigenic components were present in various proportions in these fractions.

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