Achromopeptidase for rapid lysis of oral anaerobic Gram‐positive rods.
- 1 June 1988
- journal article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 3 (2) , 86-88
- https://doi.org/10.1111/j.1399-302x.1988.tb00088.x
Abstract
A rapid and simple method for lysing anerobic Gram‐positive rods is described. It is based on the simultaneous action of lysozyme and achromopeptidase (each at 5 mg/g wet weight of cells), followed by the action of SDS (0.2%). The efficiency of the bacterial lysis is measured by the yield of DNA obtained with Marmur's modified procedure. This method permits effective lysis of small amounts of anaerobic rods (200 mg wet weight) independent of their growth phase, and extraction of DNA for further analysis. It is particularly useful for strains with low growth such as certain species of Actinomyces and Bifidobacterium and it saves considerable time.Keywords
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