SIMPLE RADIOIMMUNOASSAY FOR TRANSFERRIN USING INSOLUBILIZED ANTITRANSFERRIN ANTIBODIES - ITS APPLICATION TO CULTURED-CELLS
- 1 January 1986
- journal article
- research article
- Vol. 107 (1) , 59-65
Abstract
A radioimmunoassay (RIA) for transferrin is described. The assay uses antitransferrin antibodies covalently coupled to the particulate support Matrex Pel 102, and is simple, sensitive, reproducible, and rapid. Transferrin measurement with this assay is independent of the degree of iron saturation of the protein. The RIA was applied to the measurement of transferrin concentrations in a variety of cultured human cells. Each of 11 cells lines studied contained endogenous transferrin, but the greatest concentration was found in Chang liver cells. Red blood cells were used as a negative control.This publication has 3 references indexed in Scilit:
- Placental transferrin receptor. Evaluation of the presence of endogenous ligand on specific binding.Journal of Biological Chemistry, 1983
- Radioimmunochemical measurement of the transferrin receptor in human trophoblast and reticulocyte membranes with a specific anti-receptor antibody.Proceedings of the National Academy of Sciences, 1981
- Control of serum protein production in hepatocyte hybridomas: immortalization and expression of normal hepatocyte genes.Proceedings of the National Academy of Sciences, 1980