Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae
- 1 September 1987
- Vol. 3 (3) , 175-185
- https://doi.org/10.1002/yea.320030305
Abstract
The cDNA copies of the two endo‐β‐1,4‐glucanase genes, egl1 and egl3, from the filamentous fungus Trichoderma reesei were expressed in yeast Saccharomyces cerevisiae under the control of the yeast phosphoglycerate kinase gene promoter. Active EGI and EGIII enzyme was produced and secreted by yeast into the growth medium. The recombinant EGI enzyme was larger and more heterogeneous in size than the native enzyme secreted by Trichoderma, due to differences in the extent of N‐glycosylation between these two organisms. The morphology of the yeast cells producing EGI or EGIII was clearly different from control strain.Keywords
This publication has 33 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Cloning, Characterization and Expression in Saccharomyces Cerevisiae of Endoglucanase I from Trichoderma ReeseiNature Biotechnology, 1987
- Limited proteolysis of the cellobiohydrolase I from Trichoderma reeseiFEBS Letters, 1986
- Separation of yeast protoplasts from membrane ghosts using an aqueous two-phase systemBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I geneGene, 1986
- Detection and differentiation of cellulase components using low molecular mass fluorogenic substratesFEBS Letters, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Trichoderma reesei cellulasesTrends in Biotechnology, 1983
- Synthesis of the Yeast Cell Wall and its RegulationAnnual Review of Biochemistry, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970