Excision Repair of Gamma-ray-induced Alkali-stable DNA Lesions with the Help of γ-endonuclease fromMicrococcus Luteus
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 35 (3) , 253-263
- https://doi.org/10.1080/09553007914550291
Abstract
γ-Endonuclease Y, an enzyme that hydrolyses phosphodiester bonds at alkalistable lesions in γ-irradiated (N2, tris buffer) DNA, has been partially purified from Micrococcus luteus. The enzyme has a molecular weight of about 19 000, induces single-strand breaks with 3′OH-5′PO4 termini and contains endonuclease activity towards DNA treated with 7-bromomethylbenz(a)anthracene. γ-Endonuclease Y induces breaks in OsO4-treated poly(dA—dT) and apparently is specific towards γ-ray-induced base lesions of the t′ type. The complete excision repair of γ-endonuclease Y substrate sites has been performed in vitro by γ-endonuclease Y, DNA polymerase and ligase.This publication has 24 references indexed in Scilit:
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