Dulcitol–sucrose–salicin–iron–urea agar (DSSIU)—a new medium for differential diagnosis of Salmonellae

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Abstract
A new dulcitol–salicin–sucrose–iron–urea medium is described which, on the basis of a single-tube culture, tests the fermentation of three sugars, H2S production and urea hydrolysis.On this medium 91% of 228 Salmonellae gave easily recognized characteristic reactions, simulated only by some strains of theBethesda-Ballerupgroup. Proteus strains and most paracolon strains gave equally characteristic pictures.The reactions of some other Enterobacteria are described. It is suggested that the medium is good enough to deserve extended trial in routine work.