Five-Year Preservation ofFusariumSpecies on Silica Gel and Soil

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Abstract
Cultures of 17 Fusarium spp. (461 isolates) that were prepared by the single-spore and hyphal-tip method were stored on both silica gel and sterile soil for 5 yr at 4.5 .+-. 0.5 C. Species tested included: F. acuminatum, F. avenaceum, F. culmorum, F. dimerum, F. equiseti, F. graminearum, F. lateritium, F. merismoides, F. moniliforme, F. oxysporum, F. poae, F. proliferatum, F. sambucinum, F. semitectum, F. solani, F. sporotrichioides, and F. subglutinans. Survival on silica gel after 3, 4 and 5 yr averaged 94, 90 and 89%, respectively, compared with survival on sterile soil, which averaged 95, 93 and 94%, respectively. Storage on silica gel is quick and easy; fungi do not grow in storage; and repeated isolations can be made from a single tube. Dense (turbid) suspensions of conidia are needed initially to ensure long-term preservation on silica gel.

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