Distinguishing Rose Cultivars by Polyacrylamide Gel Electrophoresis. II. Isoenzyme Variation Among Cultivars1

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Abstract
Protein and active enzymes were extracted from leaves of 8 rose cultivars. Extracts were run on polyacrylamide gels and stained for total protein, peroxidase, esterase, malate dehydrogenase, cytochrome oxidase, phenoloxidase, and polyphenoloxidase. Three leaf positions on each cultivar were sampled in an attempt to determine a sampling standard. Because of differences in growth habit among the cultivars, and different optimum locations for the enzymes, a standard location could not be set. Banding data were tabulated and used to prepare coefficient of similarity values and flow charts showing separations. All cultivars could be distinguished by differences in their enzyme banding patterns if results from several systems were employed.

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