Characteristics of Escherichia coli B Resistant to Cobaltous Ion

Abstract
The cobalt-resistant mutant (resistant up to 1000 μg Co/ml) was obtained from E. coli B-ATCC11246 by means of incubating the parent cells in the media which contains cobalt. The mutant produced by this way is resistant only to cobalt, but not to other metals such as Mg2+, Ca2+, Cr3+, Cr2O72−, Mn2+, Fe2+, Fe8+, Ni2+, Cu2+, Zn2+ Cd2+ and Hg2+, and specifically suppresses Co2+ uptake. The difference in membrane proteins between the parent and resistant cells is observed. The granules densely stained are appeared in the parent cells incubated with Co2+ under the image of electron microscope.

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