Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts
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Open Access
- 31 October 2001
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 31 (11) , 3153-3164
- https://doi.org/10.1002/1521-4141(200111)31:11<3153::aid-immu3153>3.0.co;2-0
Abstract
The glycosylphosphatidylinositol‐anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co‐receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcγ‐receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co‐clustering with Toll‐like receptor 4, Fcγ‐RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand‐specific cellular responses initiated by CD14.Keywords
This publication has 28 references indexed in Scilit:
- Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopyThe EMBO Journal, 2000
- C(−260)→T Polymorphism in the Promoter of the CD14 Monocyte Receptor Gene as a Risk Factor for Myocardial InfarctionCirculation, 1999
- A more mature phenotype of blood mononuclear phagocytes is induced by fluvastatin treatment in hypercholesterolemic patients with coronary heart diseaseAtherosclerosis, 1999
- Tyrosine phosphorylation and Fcγ receptor‐mediated phagocytosisFEBS Letters, 1997
- Peripheral Blood Mononuclear Phagocyte Subpopulations as Cellular Markers in HypercholesterolemiaArteriosclerosis, Thrombosis, and Vascular Biology, 1996
- Rabbit aorta and human atherosclerotic lesions hydrolyze the sphingomyelin of retained low-density lipoprotein. Proposed role for arterial-wall sphingomyelinase in subendothelial retention and aggregation of atherogenic lipoproteins.Journal of Clinical Investigation, 1996
- The Myeloid Differentiation Antigen CD14 is N‐ and O‐GlycosylatedEuropean Journal of Biochemistry, 1996
- E5531, a Pure Endotoxin Antagonist of High PotencyScience, 1995
- Comparative behavior of membrane protein-antibody complexes on motile fibroblasts: implications for a mechanism of capping.The Journal of cell biology, 1990
- Fluorescence energy transfer measurements on cell surfaces: A critical comparison of steady‐state fluorimetric and flow cytometric methodsCytometry, 1984