A Hemolytic Plaque Technique for Enumeration of Cells Producing Antibody against Penicillin

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Abstract
By conjugating penicillin on sheep erythrocytes, the localized hemolysis-in-gel technique was adapted for the study of lymphoid cells producing antibodies specific for this antibiotic. The liberation of penicillin-specific antibodies by lymphoid cells of immunized rabbits was correlated with the appearance of serum antibodies. Marked similarities were observed in the cellular response patterns detected within spleen and regional lymph nodes.